Authors:
Gould TJ, Gunewardene MS, Gudheti MV, Verkhusha VV, Yin SR, Gosse JA, Hess ST.
Summary:
Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
Source:
Nature Methods; 5(12), 1027-1030 (12/08)