Authors:
Philip J Santangelo, Aaron W Lifland, Paul Curt, Yukio Sasaki, Gary J Bassell, Michael E Lindquist, & James E Crowe Jr.
Summary:
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
Source:
Nature Methods; 6, 347-349 (04/06/09)