Authors:
Hongyan Zhou, Shili Wu, Jin Young Joo, Saiyong Zhu, Dong Wook Han, Tongxiang Lin, Sunia Trauger, Geoffery Bien, Susan Yao, Yong Zhu, Gary Siuzdak, Hans R. Scholer, Lingxun Duan, and Sheng Ding
Summary:
There is no abstract available at this time.
Introductory paragraph: Groundbreaking work demonstrated that
ectopic expression of four transcription
factors, Oct4, Klf4, Sox2, and c-Myc,
could reprogram murine somatic cells to
induced pluripotent stem cells (iPSCs) (Takahashi
and Yamanaka, 2006), andhuman
iPSCs were subsequently generated using
similar genetic manipulation (Takahashi
et al., 2007; Yu et al., 2007). To address
the safety issues arose from harboring
integrated exogenous sequences in the
target cell genome, a number of modified
genetic methods have been developed
and produced iPSCs with potentially
reduced risks (for discussion, see Yamanaka,
2009, and references therein).
However, all of the methods developed
to date still involve the use of genetic materials
and thus the potential for unexpected
genetic modifications by the exogenous
sequences in the target cells. Here
we report generation of protein-induced
pluripotent stem cells (piPSCs) from
murine embryonic fibroblasts using recombinant
cell-penetrating reprogramming
proteins. We demonstrated that
such piPSCs can long-term self-renew
and are pluripotent in vitro and in vivo.
Source:
Cell Stem Cell; (2009)