Authors:
Igor Kireev, Margot Lakonishok, Wenqiu Liu, Vishwas N Joshi, Rick Powell & Andrew S Belmont
Summary:
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP–proliferating cell nuclear antigen (PCNA).
Source:
Nature Methods; 5, 311-313 (03/16/08)