Abbott Lab Student Helps Build Silk Scaffolding for Tissue

Work supports new 3D printing technique for bioengineering.

Carnegie Mellon University (CMU) undergraduate Tahlia Altgold (pictured top) makes biomedical research using silk run more smoothly.

"Silk is a really incredible biomedical material that's been used for a long time in things like sutures,” said Ms. Altgold, a junior majoring in materials science and biomedical engineering.

McGowan Institute for Regenerative Medicine affiliated faculty member Rosalyn Abbott, PhD (pictured bottom), Assistant Professor in the Departments of Biomedical Engineering and Materials Science and Engineering at CMU, focuses on using silk in tissue engineering. In order for cells to grow, they need a support structure. Silk can provide this scaffold.

But first the raw material has to be processed. That’s where Ms. Altgold comes in. She dices discarded silkworm cocoons to boil in a sodium bicarbonate solution and separates out certain proteins for use.

"Tahlia's very thorough," Dr. Abbott said, noting how important silkworms are to multiple projects in her research lab. But it's what happens to the boiled silk solution next that really makes Dr. Abbott smile. Ms. Altgold is aiding the development of a new method of 3D printing silk proteins to create personalized new tissues for patients needing regenerative medicine.

"Silk is very biocompatible," she said. Producing silk scaffolds in a lab is nothing new but finding a way to 3D print the material without blending it with potentially problematic materials will be a breakthrough for regenerative medicine.

Silk is hard to work with at the cellular level, Dr. Abbot said. Researchers need to coerce the proteins to hold the shape they need to print, say, a new ear drum.

"We're trying to find the exact right pretreatment method for pushing the solution through the nozzle in the 3D printer so it will hold its shape after printing," Ms. Altgold said. Printing material as a 3D gel is a technique developed in the laboratory of McGowan Institute affiliated faculty member Adam Feinberg, PhD, Professor in the Departments of Biomedical Engineering and Materials Science and Engineering at CMU. "Combining this method with Dr. Abbott's expertise in biomaterials has made the whole project feel in-house, totally CMU," she said.

While the Abbott Lab was closed as part of CMU’s COVID precautions, Ms. Altgold worked remotely, honing her skills reading published literature and helping to prepare research articles for publication. The Abbott Lab is back up and running with modifications as part of CMU’s phased re-opening strategy.

"The work now is to prove our concept and refine the methodology we're working on," said Ms. Altgold, whose participation in the lab is funded by a Summer Undergraduate Research Fellowship (SURF) from CMU's Undergraduate Research Office. She is mentored by Claude King III, a PhD candidate in biomechanical engineering. "Dr. Abbott's lab does exactly the kind of work I want to pursue. I'm so interested in tissue engineering and using different cells through a materials science lens."

Dr. Abbott, likewise, said Ms. Altgold is exactly the kind of student she needs for her research.

"She's so inquisitive. The work she's doing is really difficult and she's helping us make exciting progress. I'm happy to be part of her journey," Dr. Abbot said.

Illustration: Carnegie Mellon University (Altgold) and McGowan Institute for Regenerative Medicine (Abbott)


Carnegie Mellon University News Release (09/11/20)

Bio: Dr. Rosalyn Abbott

Bio: Dr. Adam Feinberg