Authors:
Christian Frøkjær-Jensen, M Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G Moerman, & Erik M Jorgensen
Summary:
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.
Source:
Nature Methods; (05/06/10)