Authors:
Gerd Schneider, Peter Guttmann, Stefan Heim, Stefan Rehbein, Florian Mueller, Kunio Nagashima, J Bernard Heymann, Waltraud G Müller, & James G McNally
Summary:
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
Source:
Nature Methods; 7, 985-987 (11/14/10)